Refrigerated centrifuges are benchtop centrifuges that come with refrigeration features. They provide temperature controls of biomaterial during a centrifugation process. In testing procedures wherein specific conditions are needed to reproduce enzymes being prepared for study, refrigerated centrifuges tend to be more beneficial than other centrifuge types.
Key differences of refrigerated centrifuges from other centrifuges
The standout feature of refrigerated centrifuges is that they have control settings that significantly reduce temperatures during a centrifugal spinning process. These centrifuges have a temperature control unit and the rotors and racks containing the sample tubes. They are often used when collecting substances that separate rapidly, such as chloroplasts, yeast cells, and erythrocytes.
Other features of this kind of centrifuge are:
- Sealed centrifugation chamber – the chamber of this centrifuge is sealed off from the outside to maintain the unit’s temperature condition during operation.
- Soft start and run-down of rotor – refrigerated centrifuges start slowly and then operate at accelerating speeds until the unit’s temperature becomes stable. It also takes more time to stop the spinning so that the cooler temperatures won’t damage the rotor as it ends its run.
- Temperature stability – temperatures are kept constant throughout a spinning operation. Once it stabilises after a soft start, temperatures inside the centrifuge remain the same until the process is ended. Temperatures can also range between -20°C to -30°C during a spin.
- RCF of up to 60,000 xg – refrigerated centrifuges run at a force of up to 60,000 xg, which is ideal for separating different biological molecules.
Most centrifuges used for general laboratory testing activities do not come with temperature controls. They also don’t have refrigeration features to lower temperatures inside their units. This makes a refrigerated centrifuge more ideal for specific biomaterial separation tasks and not just mere sedimentation.